Composite
Part:BBa_K3206006:Design
Designed by: Matthew Rogan Group: iGEM19_Newcastle (2019-10-15)
Constituitively expressed ChaC gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 196
Illegal AgeI site found at 263 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This composite part is made up of a constitutively expressing promoter (BBa_J23100), RBS (BBa_B0034), ChaC CDS (BBa_K3206002) and a terminator (BBa_B0015). The composite part was made compatible with Type IIs assembly, with BsaI restriction sites and fusion sites compatible with a DVK-AF level-1 pOdd destination vector. This part was designed in benchling and ordered as a gBlock from IDT.
Source
All parts were sourced from the iGEM Registry of Standard Biological Parts.
References
Kaur, A. et al., 2017. ChaC2, an Enzyme for Slow Turnover of Cytosolic Glutathione. Journal Of Biological Chemistry, 292(2), pp.638–651.