Composite

Part:BBa_K3206006:Design

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-15)


Constituitively expressed ChaC gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 196
    Illegal AgeI site found at 263
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part is made up of a constitutively expressing promoter (BBa_J23100), RBS (BBa_B0034), ChaC CDS (BBa_K3206002) and a terminator (BBa_B0015). The composite part was made compatible with Type IIs assembly, with BsaI restriction sites and fusion sites compatible with a DVK-AF level-1 pOdd destination vector. This part was designed in benchling and ordered as a gBlock from IDT.


Source

All parts were sourced from the iGEM Registry of Standard Biological Parts.

References

Kaur, A. et al., 2017. ChaC2, an Enzyme for Slow Turnover of Cytosolic Glutathione. Journal Of Biological Chemistry, 292(2), pp.638–651.